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| Wang,Yong; Huang,Wenhua; Peng,Daizhi; Hu,Yuanbin. |
| In this work, several major procedures of the electrophoretic mobility shift assay (EMSA) were modified including swift extraction of the nucleic protein, labeling of the probe and radioautography. The modified assay required shorter time, simplified the nucleic protein extraction, increased the radioactivity of the labeling probe, skipped the tedious process of gel drying, and produced clear images. Its results were comparable, reproducible and stable. It thus has merited for wide application. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nuclear transcription factor; Electrophoretic mobility shift assay; Methodology; Modification. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400025 |
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| Xu,Jian; Wang,Yong; Yin,Jiajun; Yin,Min; Wang,Mofei; Liu,Jingang. |
| Farnesoid X receptor (FXR) and related pathways are involved in the therapeutic effect of sleeve gastrectomy for overweight or obese patients with diabetes mellitus. This study aimed to investigate the mechanism of FXR expression regulation during the surgical treatment of obese diabetes mellitus by sleeve gastrectomy. Diabetic rats were established by combined streptozotocin and high-fat diet induction. Data collection included body weight, chemical indexes of glucose and lipid metabolism, liver function, and the expression levels of musculoaponeurotic fibrosarcoma oncogene family B (MAFB), FXR, and related genes induced by sleeve gastrectomy. Chang liver cells overexpressing MAFB gene were established to confirm the expression of related genes. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes mellitus; Obesity; Sleeve gastrectomy; MAFB; FXR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700612 |
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